ON THE MECHANISM OF LIPOXYGENASE-LIKE ACTION OF BLEOMYCIN-IRON COMPLEXES

被引：9

作者：

KIKUCHI, H ^{[1
]}

TETSUKA, T ^{[1
]}

机构：

[1] JICHI MED SCH, DEPT BIOCHEM 2, MINAMI KAWACHI, TOCHIGI 32904, JAPAN

来源：

JOURNAL OF ANTIBIOTICS | 1992年 / 45卷 / 04期

关键词：

D O I：

10.7164/antibiotics.45.548

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The mechanism of lipid peroxidation catalyzed by bleomycin (BLM)-iron (Fe) complexes has been studied in vitro using sodium linoleate as a substrate. BLM-Fe(II)-O2 and BLM-Fe(III) complexes catalyze lipid peroxidation concomitantly with singlet oxygen evolution. The results from spin trapping methods and gas chromatography-mass spectroscopy (GCMS) analyses suggest that the initial step of lipid peroxidation catalyzed by BLM-Fe complexes is similar to that of soybean lipoxygenase, viz., hydrogen abstraction. However, another mechanism might be concerned in the case of BLM-Fe(II)-O2 complex. BLM-Fe complexes are also capable of enhancing singlet oxygen evolution from the hydrogen peroxide (H2O2)-hypochlorite (OCl-) system.

引用

页码：548 / 555

页数：8

共 28 条

[1]

AUST SD, 1982, FREE RADICALS BIOL, V5, P1

[2]

BRAUGHLER JM, 1986, J BIOL CHEM, V261, P282

[3]

BRITIGAN BE, 1986, J BIOL CHEM, V261, P4426

[4]

BURGER RM, 1981, J BIOL CHEM, V256, P1636

[5] LIPID-PEROXIDATION BY BLEOMYCIN-IRON COMPLEXES INVITRO [J].

EKIMOTO, H ;

TAKAHASHI, K ;

MATSUDA, A ;

TAKITA, T ;

UMEZAWA, H .

JOURNAL OF ANTIBIOTICS, 1985, 38 (08) :1077-1082

[6] SPIN TRAPPING OF SUPEROXIDE AND HYDROXYL RADICAL - PRACTICAL ASPECTS [J].

FINKELSTEIN, E ;

ROSEN, GM ;

RAUCKMAN, EJ .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1980, 200 (01) :1-16

[7]

FUJITA Y, 1987, FREE RADICALS CLIN B, V2, P39

[8]

GREEN MJ, 1984, METHOD ENZYMOL, V105, P3

[9] EVIDENCE FOR INVOLVEMENT OF IRON IN ADP-ACTIVATED PEROXIDATION OF LIPIDS IN MICROSOMES + MITOCHONDRIA [J].

HOCHSTEIN, P ;

ERNSTER, L ;

NORDENBRAND, K .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1964, 14 (04) :323-&

[10]

KANOFSKY JR, 1986, J BIOL CHEM, V261, P3546

← 1 2 3 →