A COMPARATIVE-STUDY OF HLA-DRB1 TYPING BY STANDARD SEROLOGY AND HYBRIDIZATION OF NONRADIOACTIVE SEQUENCE-SPECIFIC OLIGONUCLEOTIDE PROBES TO PCR-AMPLIFIED DNA

被引:53
作者
MICKELSON, E [1 ]
SMITH, A [1 ]
MCKINNEY, S [1 ]
ANDERSON, G [1 ]
HANSEN, JA [1 ]
机构
[1] UNIV WASHINGTON,SCH MED,DEPT MED,SEATTLE,WA 98195
来源
TISSUE ANTIGENS | 1993年 / 41卷 / 02期
关键词
HLA-DR SEROLOGY; OLIGOTYPING; POLYMERASE CHAIN REACTION;
D O I
10.1111/j.1399-0039.1993.tb01984.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
A double-blind study was carried out to evaluate the relative performance and reliability of the PCR/SSOP assay compared to conventional serological typing in identifying HLA-DR alleles. A total of 268 consecutive samples were entered into the study. In 14 (5.2%) of the cases, HLA-DR serology could not be performed due to poor cell viability, while in seven (2.6%) of the cases, PCR/SSOP typing could not be performed due to poor amplification or to contamination with exogenous DNA. Among samples that were successfully typed by both methods, serologic typing correctly identified 455/465 (97.9%) DR antigens, while PCR/SSOP correctly identified 464/465 (99.8%) DR alleles (p=0.0117, McNemar's test). The majority of discrepancies in serologic typing resulted from a lack of discriminative alloantisera to identify DR6 or DR103. For the overall sample set (N=268), serology provided accurate results in 244 (91.0%) cases, while PCR/SSOP provided accurate results in 260 (97.0%) cases (p=0.0037). The results of this study demonstrate that PCR/SSOP typing for HLA-DRB1 alleles provides results that are equal to or surpass serological typing for HLA-DR antigens. In addition, the PCR/SSOP approach offers the advantages of better reagent availability, lower cost, more rapid turn-around time, and greater accuracy, all of which would warrant its use as an HLA typing method of choice.
引用
收藏
页码:86 / 93
页数:8
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