RECOMBINANT RABBIT HEMORRHAGIC-DISEASE VIRUS CAPSID PROTEIN EXPRESSED IN BACULOVIRUS SELF-ASSEMBLES INTO VIRUS-LIKE PARTICLES AND INDUCES PROTECTION

被引：146

作者：

LAURENT, S

VAUTHEROT, JF

MADELAINE, MF

LEGALL, G

RASSCHAERT, D

机构：

[1] INRA, UNITE VIROL & IMMUNOL MOLEC, F-78350 JOUY EN JOSAS, FRANCE

[2] CTR NATL ETUD VET & ALIMENTAIRES, F-22440 PLOUFRAGAN, FRANCE

来源：

JOURNAL OF VIROLOGY | 1994年 / 68卷 / 10期

关键词：

D O I：

10.1128/JVI.68.10.6794-6798.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

VP60, the unique component of rabbit hemorrhagic disease virus capsid, was expressed in the baculovirus system. The recombinant VP60, released in the supernatant of infected insect cells, assembled without the need of any other viral component to form viruslike particles (VLPs), structurally and immunologically indistinguishable from the rabbit hemorrhagic disease virion. Intramuscular vaccination of rabbits with the VLPs conferred complete protection in 15 days; this protection was found to be effective from the fifth day after VLP injection and was accompanied by a strong humoral response.

引用

页码：6794 / 6798

页数：5

共 34 条

[1] EXPRESSION OF 2 BOVINE ROTAVIRUS NONSTRUCTURAL PROTEINS (NSP-2, NSP-3) IN THE BACULOVIRUS SYSTEM AND PRODUCTION OF MONOCLONAL-ANTIBODIES DIRECTED AGAINST THE EXPRESSED PROTEINS
APONTE, C
MATTION, NM
ESTES, MK
CHARPILIENNE, A
COHEN, J
[J]. ARCHIVES OF VIROLOGY, 1993, 133 (1-2) : 85 - 95
[2] BEAMES B, 1991, BIOTECHNIQUES, V11, P378
[3] INVITRO TRANSLATION OF A SUBGENOMIC MESSENGER-RNA FROM PURIFIED VIRIONS OF THE SPANISH FIELD ISOLATE AST/89 OF RABBIT HEMORRHAGIC-DISEASE VIRUS (RHDV)
BOGA, JA
MARIN, MS
CASAIS, R
PRIETO, M
PARRA, F
[J]. VIRUS RESEARCH, 1992, 26 (01) : 33 - 40
[4] MONOCLONAL-ANTIBODIES TO FELINE CALICIVIRUS
CARTER, MJ
ROUTLEDGE, EG
TOMS, GL
[J]. JOURNAL OF GENERAL VIROLOGY, 1989, 70 : 2197 - 2200
[5] DETURISO JAL, 1992, J VIROL, V66, P2748
[6] GAGNIERE JP, COMMUNICATION
[7] PROTEINS OF NORWALK VIRUS
GREENBERG, HB
VALDESUSO, JR
KALICA, AR
WYATT, RG
MCAULIFFE, VJ
KAPIKIAN, AZ
CHANOCK, RM
[J]. JOURNAL OF VIROLOGY, 1981, 37 (03) : 994 - 999
[8] HERBERT TP, 1993, 9TH INT C VIR, P25
[9] SEQUENCE AND GENOMIC ORGANIZATION OF NORWALK VIRUS
JIANG, X
WANG, M
WANG, KN
ESTES, MK
[J]. VIROLOGY, 1993, 195 (01) : 51 - 61
[10] LINEARIZATION OF BACULOVIRUS DNA ENHANCES THE RECOVERY OF RECOMBINANT VIRUS EXPRESSION VECTORS
KITTS, PA
AYRES, MD
POSSEE, RD
[J]. NUCLEIC ACIDS RESEARCH, 1990, 18 (19) : 5667 - 5672

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