HIGH NITRIC-OXIDE SYNTHASE ACTIVITY IN ENDOTHELIAL-CELLS IN ULCERATIVE-COLITIS

被引：24

作者：

IWASHITA, E

MIYAHARA, T

HINO, K

TOKUNAGA, T

WAKISAKA, H

SAWAZAKI, Y

机构：

[1] Second Department of Internal Medicine, National Defense Medical College, Saitama, 359

[2] First Department of Anatomy, National Defense Medical College, Saitama, 359

来源：

JOURNAL OF GASTROENTEROLOGY | 1995年 / 30卷 / 04期

关键词：

NITRIC OXIDE SYNTHASE; ENDOTHELIAL CELLS; ULCERATIVE COLITIS;

D O I：

10.1007/BF02347578

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Endothelial nitric oxide (NO) synthase, a unique NO synthase (NOS) isoform that is expressed constitutively by the vascular endothelium both in vivo and in vitro, is believed to be essential to systemic and/or local vascular integrity. NOS expression by endothelial cells may indicate vascular activation. We successfully established a simple method for the culture of microvascular endothelial cells from a small amount of tissue and investigated ulcerative colitis (UC), in which condition vascular factors have not been studied extensively. We cultured endothelial cells from the mesenteries of surgical patients with UC and assayed NOS activity by reduced nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry. Strong NOS activity was demonstrated in the cells from all UC patients (5/5), whereas no activity was detected in the cells from human umbilical veins and the mesenteries of colon cancer patients (0/10 and 0/5, respectively). This strong NOS activity was not diminished by incubation with a high concentration of glucocorticoid, suggesting that it was constitutive. These results indicate a close relationship of vascular activation (high NOS activity) with the pathogenesis of UC.

引用

页码：551 / 554

页数：4

共 17 条

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