Density-Dependent Inhibition of Cell Growth by Transforming Growth Factor-beta 1 in Normal Human Fibroblasts

被引：63

作者：

Paulsson, Ylva ^{[1
]}

Beckmann, M. Patricia ^{[2
]}

Westermark, Bengt ^{[1
]}

Heldin, Carl-Henrik ^{[3
]}

机构：

[1] Univ Hosp, Dept Pathol, S-75185 Uppsala, Sweden

[2] NCI, Lab Cellular & Mol Biol, Bethesda, MD 20892 USA

[3] Ludwig Inst Canc Res, Biomed Ctr, S-75123 Uppsala, Sweden

来源：

GROWTH FACTORS | 1988年 / 1卷 / 01期

关键词：

density-dependent cell growth; growth factors; TGF-beta;

D O I：

10.3109/08977198809000243

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We report here that transforming growth factor-beta 1 (TGF-beta 1) inhibits platelet-derived growth factor (PDGF)-induced DNA synthesjs in normal human fibroblasts in a cell density-dependent manner; no inhibition was seen in sparse cultures, approximately 50% inhibition in confluent cell cultures, and an almost total inhibition in dense cultures. The PDGF-inducible genes c- myc and c-fos were induced also by TGF-beta 1. Simultaneous addition of TGF-beta 1 and PDGF resulted in sustained, rather than transient, expression of c-fos mRNA; c-fos mRNA was detected as long as 24 hr after addition of PDGF and TFG-beta 1. TGF-beta 1 also induced mRNA for the A chain, but not the B chain, of PDGF. Conversely, PDGF induced TGF-beta 1 mRNA in sparse but not in dense cultures. These data indicate the existence of a complex interdependent regulation of PDGF and TGF-beta mRNA expression which is influenced by the cell density.

引用

页码：19 / 27

页数：9

共 46 条

[1] INCREASED SECRETION OF TYPE-BETA TRANSFORMING GROWTH-FACTOR ACCOMPANIES VIRAL TRANSFORMATION OF CELLS [J].