INDIRECT COUPLING OF ORGANIC ANION SECRETION TO SODIUM IN TELEOST (PARALICHTHYS-LETHOSTIGMA) RENAL TUBULES

被引:52
作者
MILLER, DS
PRITCHARD, JB
机构
[1] NIEHS, CELLULAR & MOLEC PHARMACOL LAB, COMPARAT MEMBRANE PHARMACOL SECT, RES TRIANGLE PK, NC 27709 USA
[2] DUKE UNIV, MARINE LAB, BEAUFORT, NC 28516 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1991年 / 261卷 / 06期
关键词
MEMBRANE TRANSPORT; KIDNEY; PROXIMAL TUBULE; GLUTARATE; RENAL SECRETION; PARA-AMINOHIPPURATE; FLUORESCEIN; FLUORESCENCE MICROSCOPY; VIDEO IMAGING; SOUTHERN FLOUNDER; TELEOST FISH;
D O I
10.1152/ajpregu.1991.261.6.R1470
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Recent findings in both rat and crab indicate that renal accumulation of p-aminohippurate (PAH) across the basolateral membrane can be coupled indirectly to the Na gradient through PAH-glutarate exchange and Na/glutarate cotransport. However, the role of this mechanism in net transepithelial PAH secretion was not examined. Therefore, proximal tubules from Southern flounder kidney were used to assess both the presence of indirect coupling in the fish and its relationship to net secretion. [C-14]glutarate uptake by proximal tubular masses was concentrative, Na dependent, and Li inhibitable. Glutarate efflux from preloaded masses was stimulated by addition of PAH to the medium. Thus flounder tubules exhibited both Na/glutarate uptake and glutarate-PAH exchange. Furthermore, steady-state [H-3]PAH accumulation was increased 50% by 10-50-mu-M glutarate, and this increase was abolished by Li, indicating indirect coupling of PAH entry to Na. To determine whether indirect coupling affected net secretion as well as tissue accumulation, the steady-state accumulation of an anionic dye, fluorescein (FL), was measured in individual renal tubules by use of epifluorescence microscopy and video-image analysis. FL accumulated in tubules to levels that were 20-40 times higher than the medium. In most fish, luminal fluoresence was measurably higher than cellular, and uptake in both compartments was markedly reduced by PAH and Li. Moreover, FL accumulation in cells and lumina was increased by 70-100% when 50-mu-M glutarate was added to the bathing medium. Thus glutarate not only stimulated uphill FL entry into the cells, but also stimulated active secretion into the tubular lumen.
引用
收藏
页码:R1470 / R1477
页数:8
相关论文
共 19 条
[1]   SODIUM-DEPENDENT DICARBOXYLATE TRANSPORT IN RAT RENAL BASOLATERAL MEMBRANE-VESICLES [J].
BURCKHARDT, G .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1984, 401 (03) :254-261
[2]  
CHATSUDTHIPONG V, 1991, FASEB J, V5, pA1470
[3]  
DAWSON MA, 1990, J PHARMACOL EXP THER, V254, P39
[4]   PARA-AMINOHIPPURIC ACID TRANSPORT INTO BRUSH-BORDER VESICLES ISOLATED FROM FLOUNDER KIDNEY [J].
EVELOFF, J ;
KINNE, R ;
KINTER, WB .
AMERICAN JOURNAL OF PHYSIOLOGY, 1979, 237 (04) :F291-F298
[5]   USE OF ISOLATED RENAL TUBULES FOR THE EXAMINATION OF METABOLIC PROCESSES ASSOCIATED WITH ACTIVE CELLULAR TRANSPORT [J].
FORSTER, RP ;
TAGGART, JV .
JOURNAL OF CELLULAR AND COMPARATIVE PHYSIOLOGY, 1950, 36 (02) :251-270
[6]   CHLORPHENOL RED INFLUX AND EFFLUX - MICROSPECTROPHOTOMETRY OF FLOUNDER KIDNEY TUBULES [J].
KINTER, WB .
AMERICAN JOURNAL OF PHYSIOLOGY, 1966, 211 (05) :1152-&
[7]  
KINTER WB, 1975, FORTSCHR ZOOL, V23, P223
[8]  
MILLER DS, 1981, J PHARMACOL EXP THER, V219, P428
[10]  
PRITCHARD JB, 1990, J PHARMACOL EXP THER, V255, P969