HETEROGENEOUS EXPRESSION OF C-MYB PROTEIN IN HUMAN LEUKEMIA DETECTED BY SIMULTANEOUS 2 COLOR FLOW CYTOMETRIC ANALYSIS

被引：8

作者：

TESCH, H

MICHELS, M

JUCKER, M

PAHL, I

KLEIN, S

BADING, H

MOELLING, K

DIEHL, V

机构：

[1] NEUROL CLIN,W-8700 WURZBURG,GERMANY

[2] MAX PLANCK INST MOLEC GENET,W-1000 BERLIN,GERMANY

来源：

LEUKEMIA RESEARCH | 1992年 / 16卷 / 03期

关键词：

LEUKEMIA; FLOW CYTOMETRY; ONCOGENE; C-MYB;

D O I：

10.1016/0145-2126(92)90064-E

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The expression of c-myb mRNA and protein was analyzed in fresh leukemic cells by Northern-blot analyses and by immunofluorescent staining using monoclonal c-myb specific antibodies. Staining of the cells was evaluated by flow cytometry. The results demonstrate c-myb mRNA expression predominantly in acute lymphocytic leukemia (ALL, 4/4 cases), acute myeloic leukemia (AML, 17/17) and chronic myeloic leukemia (CML, 12/12) but rarely in chronic lymphocytic leukemia (CLL, 1/17). Immunofluorescent analyses revealed expression of c-myb protein in the nucleus of ALL (5/7) and AML (9/9) with a good correlation of c-myb-positive cells and with the number of proliferating (Ki67-positive) blast cells.

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页码：265 / &

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