EFFECTS OF CADMIUM ON LYMPHOCYTE-PROLIFERATION AND MACROPHAGE ACTIVATION IN CATFISH, ICTALURUS-MELAS

被引：11

作者：

ALBERGONI, V

VIOLA, A

机构：

[1] Department of Biology, University of Padova, 35125 Padova

来源：

FISH & SHELLFISH IMMUNOLOGY | 1995年 / 5卷 / 04期

关键词：

CADMIUM; LYMPHOCYTES; MACROPHAGES; CATFISH;

D O I：

10.1006/fsim.1995.0029

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The effects of cadmium (Cd) (in vitro and in vivo) on phytohaemoagglutinin(PHA)- or lipopolysaccharide(LPS)-induced lymphocyte proliferation were investigated in the catfish, Ictalurus melas. The effects of Cd on macrophage activation by Concanavallina A or Saccharomyces cerevisiae, as measured by superoxide (O-2(-)) production, were investigated. Dose-independent (except at 2 mu M Cd) inhibition of T cell stimulation and dose-dependent inhibition of B cell stimulation, by lymphocytes exposed in vitro to 2-40 mu M Cd, was found. In vivo, 20 mu g l(-1) Cd inhibited both PHA- and LPS-induced lymphocyte proliferation, at 4 days of exposure and throughout the duration of the experiment. During the first week of exposure, Cd significantly decreased spontaneous H-3-methyl-thymidine uptake from unstimulated lymphocytes; however, 15 days after exposure unstimulated cells recovered their basal level of spontaneous thymidine uptake. In vitro 2-40 mu M Cd enhanced O-2(-) production by activated macrophages, while the addition of 50-100 mu M Cd had no significant effects.

引用

页码：301 / 311

页数：11

共 29 条

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