PREPARATION OF STABLE INTERMEDIATE-PURITY FACTOR-VIII CONCENTRATE WITH A NOTE ON HIGH-PURITY FACTOR-VIII

Abstract. Cryoprecipitate prepared by a rapid thawing technique was pooled in batches of 600–720 donor units and washed with ice‐cold Tris‐citrate‐NaCl solution. After dissolving at 37°C, it was adsorbed with Al(OH)3 and kaolin, and cleared by centrifugation. The supernatant, diluted with 5% dextrose was passed repeatedly through a bed of Celite, filtered through a 293 mm × 0.3 μm membrane disc and lyophilized. Typical composition was 15 U ml‐1 factor VIII, and 40 mg ml‐1 protein with a yield of 300 U/l of starting plasma. The crude factor VIII concentrate was also a suitable material for preparation of high‐purity factor VIII by controlled pore glass chromatography. © 1979 Blackwell Publishing Ltd