PURIFICATION AND BIOCHEMICAL CHARACTERISTICS OF OCCLUSION BODY OF PENAEUS-MONODON-TYPE BACULOVIRUS (MBV)

被引：7

作者：

CHANG, PS

WANG, YC

LO, CF

KOU, GH

CHEN, SN

机构：

来源：

FISH PATHOLOGY | 1992年 / 27卷 / 03期

关键词：

D O I：

10.3147/jsfp.27.127

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

In the present study, occlusion bodies (OBs) of Penaeus monodon-type baculovirus (MBV) were collected from infected juvenile P. monodon and then the OBs were purified by centrifugation for 30 min at a speed of 100,000 x g using 35-65% (w/w) sucrose gradient. Analysis of polypeptides of MBV occlusion bodies showed 12 polypeptides in SDS-PAGE gel. It was also concluded that the major band with molecular mass of 62 k dalton may be derived from protein of polyhedrin.

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页码：127 / 130

页数：4

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