THE PROMOTER ELEMENT GTACAAG OF THE SGA AND STA2 GENES IS A POSSIBLE TARGET SITE FOR REPRESSION BY THE STA10 GENE-PRODUCT FROM SACCHAROMYCES-CEREVISIAE

被引：3

作者：

CLAROS, MG ^{[1
]}

DELPOZO, L ^{[1
]}

ABARCA, D ^{[1
]}

JIMENEZ, A ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,CTR BIOL MOLEC,CANTO BLANCO,E-28049 MADRID,SPAIN

来源：

FEMS MICROBIOLOGY LETTERS | 1992年 / 92卷 / 01期

关键词：

GLUCOAMYLASE GENES; STA10 REPRESSOR GENE; REGULATORY ELEMENT; BETA-GALACTOSIDASE ACTIVITY; SACCHAROMYCES-CEREVISIAE;

D O I：

10.1016/0378-1097(92)90541-U

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The SGA and STA2 genes that, respectively, encode the intra- and extracellular glucoamylases of Saccharomyces cerevisiae are coregulated negatively, at the level of transcription, by the STA10 gene. This finding was re-examined by determining the effects of STA10 on the expression of gene constructs containing different fragments from the SGA and STA2 promoter regions fused to the lacZ gene. Repression was observed only for promoter fragments carrying the sequence GTACAAG indicating that this element is responsible for the coregulation of SGA and STA2 by STA10.

引用

页码：57 / 62

页数：6

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