EFFECT OF PH ON QUERCETIN-INDUCED SUPPRESSION OF HEAT-SHOCK GENE-EXPRESSION AND THERMOTOLERANCE DEVELOPMENT IN HT-29 CELLS

被引：30

作者：

LEE, YJ ^{[1
]}

CURETTY, LL ^{[1
]}

HOU, ZZ ^{[1
]}

KIM, SH ^{[1
]}

KIM, JH ^{[1
]}

CORRY, PM ^{[1
]}

机构：

[1] HENRY FORD HOSP,DEPT RADIAT ONCOL,DETROIT,MI 48202

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 186卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)90863-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

When cells were heated for 15 min at 45°C, they became thermotolerant to a second heat exposure at 45°C. Thermotolerance developed rapidly, reached its maximum 6 hr after heat shock, and then gradually decayed. The development of thermotolerance was partially suppressed by treatment with various concentrations of quercetin (0.05-0.2 mM) at pH 7.4 after the initial heat treatment. In contrast, the drug markedly inhibited thermotolerance development at pH 6.5. Furthermore, a combination of low pH and quercetin treatment distinctively altered the expression of HSP70 gene compared with that of HSP28 or HSP90 gene. These results demonstrate a good correlation between the amount of HSP70 gene expression and development of thermotolerance. © 1992.

引用

页码：1121 / 1128

页数：8

共 16 条

[1] INHIBITION OF LACTATE TRANSPORT AND GLYCOLYSIS IN EHRLICH ASCITES TUMOR-CELLS BY BIOFLAVONOIDS [J].