PARTIAL SEQUENCE AND POLYMERASE CHAIN REACTION-MEDIATED ANALYSIS OF EXPRESSION OF THE HUMAN CYP2C18 GENE

被引：11

作者：

GED, C

BEAUNE, P

机构：

[1] INSERM U75, CHU Necker Enfants Malades, Paris Cedex 15, 75730, 156, rue de Vaugirard

来源：

PHARMACOGENETICS | 1992年 / 2卷 / 03期

关键词：

D O I：

10.1097/00008571-199206000-00002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe the isolation of the human CYP2C18 gene, a new member in the complex CYP2C subfamily, associated with the genetically-determined polymorphism for (S)-mephenytoin hydroxylase. The 5' end of CYP2C18 gene was isolated from a human genomic library using a probe derived from the CYP2C10 cDNA and the 5' flanking region, exons 1 to 4 and intron-exon junctions were sequenced. With respect to intron-exon boundaries, the partial gene structure was identical to that of rat and rabbit CYP2C genes. Consensus sequences for putative 'glucocorticoid responsive elements' were observed in the 5' flanking region and in intron 1, an interesting feature in a so-called constitutively-expressed gene subfamily. The knowledge of CYP2C gene sequences is a prerequisite to genomic analysis by PCR techniques. Using oligonucleotides derived from the gene sequence, we were able to specifically detect CYP2C18 mRNAs in human liver.

引用

页码：109 / 115

页数：7

共 43 条

[1]

Beaune P.H., Kremers P.G., Kaminsky L.S., De Graeve J., Albert A., Guengerich F.P., Comparison of monooxygenase activities and cytochrome P-450 isozyme concentrations in human liver microsomes, Drug Metab Dispos, 14, pp. 437-442, (1986)

[2]

Breimer D.D., Zilly W., Richter E., Influence of corticosteroid on hexobarbital and tolbutamide disposition, Clin Pharmacol Ther, 24, pp. 208-212, (1978)

[3]

Brian W.R., Srivastava P.K., Umbenhauer D.R., Lloyd R.S., Guengerich F.P., Expression of human liver cytochrome P-450 protein with tolbutamide hydroxylase activity in Saccharomyces cere- visiae, Biochemistry, 28, pp. 4993-4999, (1989)

[4]

Chan G., Kemper B., Structure of the rabbit cytochrome P450 IIC3 gene, a constitutive member of the P450 IIC subfamily, Biochemistry, 29, pp. 3743-3750, (1990)

[5]

Chomczynski P., Sacchi N., Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroforme extraction, Anal Biochem, 162, pp. 156-159, (1987)

[6]

Cresteil T., Beaune P., Kremers P., Celier C., Guengerich F.P., Leroux J.-P., Immunoquantification of epoxide hydrolase and cytochrome P450 isoenzymes in fetal and adult human liver microsomes, Eur J Biochem, 151, pp. 345-350, (1985)

[7]

Eladari M.E., Syed S.H., Guilhot S., D'Auriol L., Galibert F., On the high conservation of the human c-myc first exon, Biochem Biophys Res Commun, 140, pp. 313-319, (1986)

[8]

Freifelder D.M., Transcription, Molecular Biology, (1987)

[9]

Furuya H., Meyer U.A., Gelboin H.V., Gonralez F.J., Polymerase chain reaction-directed identification, cloning, and quantification of human CYP2C18 mRNA, Mol Pharmacol, 40, pp. 375-382, (1991)

[10]

Ged C., Umbenhauer D.R., Bellew T.M., Bork R.W., Srivastava P.K., Shinriki N., Lloyd R.S., Guengerich F.P., Characterization of cDNAs, mRNAs, and proteins related to human liver microsomal cytochrome P-450 (S)-mephenytoin 4'-hydroxylase, Biochemistry, 27, pp. 6929-6940, (1988)

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