PRESENCE OF CD8-ALPHA-CD8-BETA-POSITIVE TCR GAMMA/DELTA THYMOCYTES IN THE FETAL MURINE THYMUS AND THEIR INVITRO EXPANSION WITH INTERLEUKIN-7

被引：13

作者：

LECLERCQ, G ^{[1
]}

DESMEDT, M ^{[1
]}

PLUM, J ^{[1
]}

机构：

[1] STATE UNIV GHENT HOSP,BACTERIOL VIROL & IMMUNOL,BLOK A,DE PINTELAAN 185,B-9000 GENT,BELGIUM

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 1992年 / 22卷 / 09期

关键词：

D O I：

10.1002/eji.1830220902

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Several groups have described that a low percentage of in vitro cultured T cell receptor (TcR) gamma/delta-cells express CD8. Contrary to TcR alpha/beta-cells, however, CD8 on these TcR gamma/delta-cells was shown to be a CD8-alpha homodimer. We describe here that addition of interleukin-7 (IL-7) to a short-term in vitro culture of fetal day 14 thymic lobes in an organ culture system or of fetal day 18 fetal thymocytes in cell suspension yields CD8-beta-positive TcR gamma/delta-cells. This is not the result of IL-7-induced expression of CD8-beta on previously CD8-beta-negative cells. It is due to IL-7-induced expansion of CD8-alpha-CD8-beta-positive TcR gamma/delta-cells which are shown to be present in the starting fetal thymocyte cell population.

引用

页码：2189 / 2193

页数：5

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