PROTECTION OF MICE FROM INHALED RICIN BY VACCINATION WITH RICIN OR BY PASSIVE TREATMENT WITH HETEROLOGOUS ANTIBODY

被引：35

作者：

HEWETSON, JF ^{[1
]}

RIVERA, VR ^{[1
]}

CREASIA, DA ^{[1
]}

LEMLEY, PV ^{[1
]}

RIPPY, MK ^{[1
]}

POLI, MA ^{[1
]}

机构：

[1] USA,DIV PATHOL,FREDERICK,MD 21702

来源：

VACCINE | 1993年 / 11卷 / 07期

关键词：

RICIN; INHALATION; PROTECTION; ANTIBODY; VACCINATION;

D O I：

10.1016/0264-410X(93)90259-Z

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Mice were vaccinated subcutaneously with 25 mug kg-1 of ricin in the presence of Freund's complete adjuvant or Ribi(TM) adjuvant, followed by a boost 14 days later with 50 mug kg-1 ricin in Freund's incomplete adjuvant or Ribi adjuvant, respectively. Three subsequent boosts at 28-day intervals with 25 mug kg-1 ricin yielded high anti-ricin antibody titres as determined by ELISA. Vaccinated animals were exposed to an aerosolized LD99 dose of ricin. With the exception of one death not attributable to ricin intoxication, all vaccinated mice survived the lethal aerosol exposure. In addition, a passive protection regimen was evaluated in mice pretreated with 100 mug purified goat anti-ricin IgG administered intravenously, and then challenged with ricin intravenously. All were resistant to 125 mug kg-1 of ricin, a dose greater than 25 times the intravenous lethal dose. Mice injected intravenously with 5 mg of the same IgG were protected from a lethal aerosol challenge. These results indicated that it is possible to protect animals from inhaled ricin by vaccination or passive administration of specific antibodies.

引用

页码：743 / 746

页数：4

共 19 条

[1]

COLOMBATTI M, 1987, J IMMUNOL, V138, P3339

[2] ACUTE INHALATION TOXICITY OF T-2 MYCOTOXIN IN THE RAT AND GUINEA-PIG [J].