SEX INFLUENCE ON RENAL ALPHA(2)-ADRENERGIC RECEPTOR DENSITY IN THE SPONTANEOUSLY HYPERTENSIVE RAT

Male spontaneously hypertensive rats (SHR) have higher blood pressure than females. We compared renal alpha(2)-adrenergic receptor density among intact SHR and Wistar-Kyoto (WKY) rats of both sexes, male and female SHR gonadectomized at 4 weeks of age, and gonadectomized SHR supplemented with testosterone. Additional groups of SHR were treated with enalapril (30 mg/kg per day), an angiotensin-converting enzyme inhibitor, from 5 to 14 weeks of age. Renal ai-adrenergic receptor density was higher in males than females in both SHR and WKY rats. Female SHR and WKY rats had identical low renal alpha(2)-adrenergic receptor density. Castration of male SHR reduced the male-female differences in blood pressure and renal alpha(2)-adrenergic receptor density by 60%. Treatment with testosterone raised blood pressure and renal alpha(2)-adrenergic receptor density to the intact male levels in both gonadectomized males and females. Treatment with enalapril decreased blood pressure but not renal alpha(2)-adrenergic receptor density in both male and female SHR. We conclude that (1) both renal alpha(2)-adrenergic receptor density and blood pressure are influenced by sex in SHR and WKY, (2) renal alpha(2)-adrenergic receptor density like blood pressure is regulated by androgens, and (3) increased renal alpha(2)-adrenegic receptor density is not a consequence of high blood pressure in male SHR.