SIMULTANEOUS DETECTION OF BETA-GALACTOSIDASE ACTIVITY AND SURFACE-ANTIGEN EXPRESSION IN VIABLE HEMATOPOIETIC-CELLS

被引：21

作者：

BERGER, CN ^{[1
]}

TAN, SS ^{[1
]}

STURM, KS ^{[1
]}

机构：

[1] UNIV MELBOURNE,DEPT ANAT & CELL BIOL,PARKVILLE,VIC 3052,AUSTRALIA

来源：

CYTOMETRY | 1994年 / 17卷 / 03期

关键词：

FLOW CYTOMETRY; BETA-GALACTOSIDASE; LACZ; FDG; FLUORESCEIN-DI-BETA-D-GALACTOPYRANOSIDE; TRANSPLANTATION MARKER; X CHROMOSOME INACTIVATION;

D O I：

10.1002/cyto.990170305

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The quantitation of intracellular beta-galactosidase activity has been described for viable cells. By using the fluorogenic substrate fluorescein-di-beta-D-galactopyranoside (FDG) in conjunction with flow cytometry, the proportion of positive cells as well as the level of expression can be determined. In this paper we describe beta-galactosidase expression in lymphoid and myeloid cells from transgenic mice that widely express beta-galactosidase from an inserted lacZ transgene. Both foetal and adult haematopoietic tissues are able to express beta-galactosidase. The intracellular fluorescence reflecting beta-galactosidase activity can be readily combined with fluorescently labelled antibodies against cell surface antigens. Thus, beta-galactosidase can be used as a marker in transplantation experiments to study the development of lymphoid and myeloid precursor cells. (C) 1994 Wiley-Liss, Inc.

引用

页码：216 / 223

页数：8

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