FOOTPRINTING STUDIES ON THE SEQUENCE-SELECTIVE BINDING OF PENTAMIDINE TO DNA

被引：61

作者：

FOX, KR ^{[1
]}

SANSOM, CE ^{[1
]}

STEVENS, MFG ^{[1
]}

机构：

[1] UNIV ASTON,INST PHARMACEUT SCI,BIRMINGHAM B4 7ET,W MIDLANDS,ENGLAND

来源：

FEBS LETTERS | 1990年 / 266卷 / 1-2期

基金：

英国惠康基金;

关键词：

Drug-DNA interaction; Minor groove binding; Pentamidine;

D O I：

10.1016/0014-5793(90)81527-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The sequence-selective binding of pentamidine, an antimicrobial aromatic diamidine, has been investigated by footprinting studies on two different DNA fragments using DNase I, micrococcal nuclease and hydroxyl radical as probes. Each probe reveals drug-induced protection from cleavage in AT-rich regions. The best binding sites consist of at least 5 consecutive AT base pairs. Three or less AT pairs do not constitute a pentamidine binding site. © 1990.

引用

页码：150 / 154

页数：5

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