FROM LUC AND PHOT GENES TO THE HOSPITAL BED

被引：4

作者：

CAMPBELL, AK

SALANEWBY, G

ASTON, PJ

KALSHEKER, N

KISHI, Y

SHIMOMURA, O

机构：

[1] HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138

[2] MARINE BIOL LAB,WOODS HOLE,MA 02543

来源：

JOURNAL OF BIOLUMINESCENCE AND CHEMILUMINESCENCE | 1990年 / 5卷 / 02期

关键词：

D O I：

10.1002/bio.1170050209

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Luc gene from the firefly Photinus pyralis has been isolated by cloning it in pcDV1 PL plasmid primer and Honjo linker and the Phot gene isolated from Aequorea victoria using the polymerase chain reaction. A method has been established using SP6 RNA polymerase for transcribing and translating bioluminescent genes in vitro. It should now be possible to engineer these genes to measure intracellular ATP and the covalent modification of proteins in single, live cells, providing unique insights into the molecular basis of disease.

引用

页码：131 / 139

页数：9

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