A LIQUID RADIOCHROMATOGRAPHIC METHOD FOR FOLLOWING THE SYNTHESIS AND HYDROLYSIS OF A DOUBLY (H-3, C-14) RADIOLABELED ALKYL BETAINATE

In order to study the rate of hydrolysis of long-chain alkyl betainates in various biological environments, a technique was developed based on the introduction of different radioisotopes in the betaine and alkyl groups, respectively. The hydrolytic reaction process was then followed by liquid reversed-phase chromatography with on-line radioactivity monitoring of the reactant and products using discrete counting windows for the two radioisotopes. The 3H/14C-ratio was used to evaluate possible losses due to adsorption to the biological material. © 1990, Taylor & Francis Group, LLC. All rights reserved.