IMMOBILIZATION CAN IMPROVE THE STABILITY OF HYBRIDOMA ANTIBODY PRODUCTIVITY IN SERUM-FREE MEDIA

被引：66

作者：

LEE, GM ^{[1
]}

PALSSON, BO ^{[1
]}

机构：

[1] UNIV MICHIGAN,DEPT CHEM ENGN,HERBERT H DOW BLDG,ANN ARBOR,MI 48109

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1990年 / 36卷 / 10期

关键词：

D O I：

10.1002/bit.260361010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Hybridoma cells (S3H5/γ2bA2) were cultivated in spinner flasks with 1% serum media and serum‐free media. Monoclonal antibody productivity was maintained in 1% serum media. However, cells in serum‐free media showed a decrease in antibody productivity, and it completely disappeared in IMDM‐based low protein medium. This loss of antibody productivity was not observed when the cells were immobilized in alginate beads. In fact, immobilization enhanced the specific MAb productivity. Copyright © 1990 John Wiley & Sons, Inc.

引用

页码：1049 / 1055

页数：7

共 34 条

[1]

AARDEN LA, 1989, ANN NY ACAD SCI, V557, P192

[2] A METHOD FOR SIMULTANEOUS NUCLEAR IMMUNOFLUORESCENCE AND DNA CONTENT QUANTITATION USING MONOCLONAL-ANTIBODIES AND FLOW-CYTOMETRY [J].

CLEVENGER, CV ;

BAUER, KD ;

EPSTEIN, AL .

CYTOMETRY, 1985, 6 (03) :208-214

[3] RAPID, ONE-STEP STAINING PROCEDURES FOR ANALYSIS OF CELLULAR DNA AND PROTEIN BY SINGLE AND DUAL LASER FLOW-CYTOMETRY [J].

CRISSMAN, HA ;

STEINKAMP, JA .

CYTOMETRY, 1982, 3 (02) :84-90

[4] THE LOSS OF ANTIBODY PRODUCTIVITY IN CONTINUOUS CULTURE OF HYBRIDOMA CELLS [J].

FRAME, KK ;

HU, WS .

BIOTECHNOLOGY AND BIOENGINEERING, 1990, 35 (05) :469-476

[5] CAN CELL-CULTURE MEDIUM COSTS BE REDUCED - STRATEGIES AND POSSIBILITIES [J].

GRIFFITHS, B .

TRENDS IN BIOTECHNOLOGY, 1986, 4 (10) :268-272

[6]

Griffiths J B, 1987, Dev Biol Stand, V66, P155

[7]

GRITZMACHER CA, 1989, CRIT REV IMMUNOL, V9, P173

[8]

HANDACORRIGAN A, 1988, BIO-TECHNOL, V6, P784

[9]

HEATH CA, 1988, CS NATIONAL M LOS AN

[10] COMPLEMENTARY-DNA FOR A NOVEL HUMAN INTERLEUKIN (BSF-2) THAT INDUCES LYMPHOCYTES-B TO PRODUCE IMMUNOGLOBULIN [J].

HIRANO, T ;

YASUKAWA, K ;

HARADA, H ;

TAGA, T ;

WATANABE, Y ;

MATSUDA, T ;

KASHIWAMURA, S ;

NAKAJIMA, K ;

KOYAMA, K ;

IWAMATSU, A ;

TSUNASAWA, S ;

SAKIYAMA, F ;

MATSUI, H ;

TAKAHARA, Y ;

TANIGUCHI, T ;

KISHIMOTO, T .

NATURE, 1986, 324 (6092) :73-76

← 1 2 3 4 →