CLONING OF THE D-LACTATE DEHYDROGENASE GENE FROM LACTOBACILLUS-DELBRUECKII SUBSP BULGARICUS BY COMPLEMENTATION IN ESCHERICHIA-COLI

被引：83

作者：

BERNARD, N ^{[1
]}

FERAIN, T ^{[1
]}

GARMYN, D ^{[1
]}

HOLS, P ^{[1
]}

DELCOUR, J ^{[1
]}

机构：

[1] CATHOLIC UNIV LOUVAIN,UNITE GENET,GENET MOLEC LAB,CROIX SUD 5,BTE 6,B-1348 LOUVAIN,BELGIUM

来源：

FEBS LETTERS | 1991年 / 290卷 / 1-2期

关键词：

D-LACTATE DEHYDROGENASE; INSERTION SEQUENCE IS2; LACTOBACILLUS-DELBRUECKII SUBSP BULGARICUS; D-HYDROXYISOCAPROATE DEHYDROGENASE;

D O I：

10.1016/0014-5793(91)81226-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A strain of Escherichia coli (FMJ144) deficient for pyruvate formate lyase and lactate dehydrogenase (LDH) was complemented with a genomic DNA library from Lactobacillus delbrueckii subsp. bulgaricus. One positive clone showed LDH activity and production of D(-)lactate was demonstrated. The nucleotide sequence of the D-LDH gene (ldhA) revealed the spontaneous insertion of an E. coli insertion sequence IS2 upstream of the gene coding region. The open reading frame encoded a 333-amino acid protein, showing no similarity with known L-LDH sequences but closely related to L. casei D-hydroxyisocaproate dehydrogenase (D-HicDH).

引用

页码：61 / 64

页数：4

共 13 条

[1] CONSTRUCTION AND PROPERTIES OF A NEW INSERTION VECTOR, PJDC9, THAT IS PROTECTED BY TRANSCRIPTIONAL TERMINATORS AND USEFUL FOR CLONING OF DNA FROM STREPTOCOCCUS-PNEUMONIAE [J].

CHEN, JD ;

MORRISON, DA .

GENE, 1988, 64 (01) :155-164

[2] FROM ANALYSIS TO SYNTHESIS - NEW LIGAND-BINDING SITES ON THE LACTATE-DEHYDROGENASE FRAMEWORK .1. [J].

CLARKE, AR ;

ATKINSON, T ;

HOLBROOK, JJ .

TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (03) :101-105

[3] TOWARDS THE CONSTRUCTION OF A UNIVERSAL NAD(P)+-DEPENDENT DEHYDROGENASE - COMPARATIVE AND EVOLUTIONARY CONSIDERATIONS [J].

CLARKE, AR ;

COLEBROOK, S ;

CORTES, A ;

EMERY, DC ;

HALSALL, DJ ;

HART, KW ;

JACKSON, RM ;

WILKS, HM ;

HOLBROOK, JJ .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1991, 19 (03) :576-581

[4] SPONTANEOUS INSERTION OF AN IS2 ELEMENT INTO THE PROMOTER REGION OF THE LAC OPERON [J].

DETOGNI, P ;

FOX, ES ;

MORRISSEY, S ;

BABIOR, BM ;

LEVY, SB .

PLASMID, 1988, 20 (02) :143-147

[5]

FEENEY R, 1989, BIOCHEM BIOPH RES CO, V166, P667

[6] BACTERIAL LACTATE-DEHYDROGENASES [J].

GARVIE, EI .

MICROBIOLOGICAL REVIEWS, 1980, 44 (01) :106-139

[7]

Holbrook JJ, 1975, ENZYMES A, VXI, P191

[8]

LEBRAS G, 1991, FEMS MICROBIOL LETT, V79, P89, DOI 10.1111/j.1574-6968.1991.tb04510.x

[9] CLONING, SEQUENCING AND EXPRESSION IN ESCHERICHIA-COLI OF THE D-2-HYDROXYISOCAPROATE DEHYDROGENASE GENE OF LACTOBACILLUS-CASEI [J].

LERCH, HP ;

BLOCKER, H ;

KALLWASS, H ;

HOPPE, J ;

TSAI, H ;

COLLINS, J .

GENE, 1989, 78 (01) :47-57

[10] MUTANTS OF ESCHERICHIA-COLI DEFICIENT IN THE FERMENTATIVE LACTATE-DEHYDROGENASE [J].

MATJAN, F ;

ALAM, KY ;

CLARK, DP .

JOURNAL OF BACTERIOLOGY, 1989, 171 (01) :342-348

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