SEPARATION AND DETECTION OF PROTEIN AS A SUPRAMOLECULAR COMPLEX USING CAPILLARY ELECTROPHORESIS

被引：40

作者：

Hara, Tadashi ^{[1
]}

Okamura, Shinobu ^{[1
]}

Kato, Shigeru ^{[1
]}

Yokogi, Junichi ^{[1
]}

Nakajima, Riichiro ^{[1
]}

机构：

[1] Doshisha Univ, Fac Engn, Dept Chem Engn, Kamikyo Ku, Kyoto 602, Japan

来源：

ANALYTICAL SCIENCES | 1991年 / 7卷

关键词：

protein; separation; detection; capillary electrophoresis;

D O I：

10.2116/analsci.7.Supple_261

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

From a high performance capillary electrophoretic experiment in a phosphate buffer ( pH 3.5), Eosine Y was found for the first time to migrate together with protein in a capillary tube as a stipramolecular complex. This finding gave us not only the possibility to overcome the serious problems in protein estimation, but also the possibility to measure the Eosine Y in place of protein much more sensitively by several methods. However the results obtained so far were not so good.

引用

页码：261 / 264

页数：4