CHARACTERIZATION OF ENDOTHELINS AS CHEMOATTRACTANTS FOR HUMAN NEUTROPHILS

被引：37

作者：

WRIGHT, CD

CODY, WL

DUNBAR, JB

DOHERTY, AM

HINGORANI, GP

RAPUNDALO, ST

机构：

[1] WARNER LAMBERT PARKE DAVIS,PARKE DAVIS PHARMACEUT RES,DEPT IMMUNOPATHOL,ANN ARBOR,MI 48105

[2] WARNER LAMBERT PARKE DAVIS,PARKE DAVIS PHARMACEUT RES,DEPT CHEM,ANN ARBOR,MI 48105

[3] WARNER LAMBERT PARKE DAVIS,PARKE DAVIS PHARMACEUT RES,DEPT CARDIOVASC THERAPEUT,ANN ARBOR,MI 48105

来源：

LIFE SCIENCES | 1994年 / 55卷 / 21期

关键词：

ENDOTHELIN; HUMAN NEUTROPHIL; CHEMOTAXIS;

D O I：

10.1016/0024-3205(94)00330-0

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

The chemotactic response of human neutrophils to endothelins (ET) and ET-derived peptides was examined. ET-1, ET-2, and ET-3 elicited maximum responses at 10(-7), 3.3 x 10(-8) and 10(-7) M, respectively. Relative activities of the peptides at their optimal concentrations were: ET-1, ET-2 > ET-3. The chemotactic activity of ET-1 was localized to its Leu(6)-Met(7)-Asp(8) segment. Conformation of the disulfide-linked Cys(3) -Cys(11) loop appears to be critical for proper orientation of the chemotactic epitope. In comparison, ET-1 failed to stimulate the neutrophil respiratory burst, degranulation or arachidonic acid metabolism. These results demonstrate the selective chemoattractant activity of endothelins for human neutrophils.

引用

页码：1633 / 1641

页数：9

共 31 条

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