STUDIES ON RIBONUCLEASE PRODUCED BY RHIZOPUS SP .I. CRYSTALLIZATION AND SOME PROPERTIES OF RIBONUCLEASE

被引：51

作者：

TOMOYEDA, M

ETO, Y

YOSHINO, T

机构：

[1] Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University, Kakamigahara, Gifu

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1969年 / 131卷 / 01期

关键词：

D O I：

10.1016/0003-9861(69)90121-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A ribonuclease (RNase) of Rhizopus sp. was crystallized from 0.5-0.6 saturated ammonium sulfate at pH 5.0. The crystal was found to be homogeneous electrophoretically and ultracentrifugally. The optimum pH of the RNase was 5.0 and optimum temperature was found in the range of 50-55 ° and it was inhibited by the divalent metal ions such as Zn++, Cu++ and Hg++, but not affected by ethylenediaminetetraacetic acid (EDTA). When the yeast RNA was digested with the enzyme, adenylic acid and guanylic acid were produced at a faster rate than the other mononucleotides. © 1969.

引用

页码：191 / &

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[1] STUDIES ON EXTRACELLULAR RIBONUCLEASES OF USTILAGO SPHAEROGENA - CHARACTERIZATION OF SUBSTRATE SPECIFICITY WITH SPECIAL REFERENCE TO PURINE-SPECIFIC RIBONUCLEASES [J].