ANALYTICAL STUDIES OF MARIDOMYCIN .1. HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF MARIDOMYCINS AND SOME OTHER MACROLIDE ANTIBIOTICS

Maridomycins and their acyl derivatives (9-propionyl, 2′-propionyl and 9,2′-dipropionyl maridomycins) were separated quantitatively into six components by high-performance liquid chromatography using a Corasil I column (200 cm × 2 mm I.D.) with a mixed sovent (upper layer of n-hexane, diisopropyl ether, ethanol and water) as the eluent. A linear relationship was found between the logarithm of the capacity factors (k′) and the logarithm of the alkyl carbon numbers in the acyl group at position 4′'. This relationship led to the discovery of a new component of maridomycin (3-propionyl-4′'-n-butyryl analogue) in a crude sample. A similar relationship was found on chromatographic separation of some other macrolide antibiotics such as leucomycin, carbomycin A and B groups, under similar conditions. © 1979.