UVA-INDUCED LIPID-PEROXIDATION IN CULTURED HUMAN FIBROBLASTS

被引：149

作者：

MORLIERE, P

MOYSAN, A

SANTUS, R

HUPPE, G

MAZIERE, JC

DUBERTRET, L

机构：

[1] MUSEUM NATL HIST NAT, PHYSICOCHIM ADAPTAT BIOL LAB, INSERM, U312, F-75231 PARIS 05, FRANCE

[2] UNIV PARIS 06, BIOCHIM LAB, F-75571 PARIS 12, FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1084卷 / 03期

关键词：

UVA LIGHT; ENDOGENOUS PHOTOSENSITIZER; PEROXIDATION; MEMBRANE; FIBROBLAST;

D O I：

10.1016/0005-2760(91)90068-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The UVA irradiation of cultured human fibroblasts leads to the formation and to the release of thiobarbituric acid-reactive substances in the supernatant. The major thiobarbituric acid-reactive substance is identified by fluorescence spectroscopy and HPLC, as malondialdehyde or malondialdehyde-forming substances under the thiobarbituric acid assay conditions. Malondialdehyde formation strongly suggests a UVA-induced lipid peroxidation. Lipid peroxidation is also supported by the inhibitory effect of D,L-alpha-tocopherol, the well-known chain breaking antioxidant, by the additional malondialdehyde formation in the dark after the photooxidative stress and by membrane damage revealed by lactate dehydrogenase leakage.

引用

页码：261 / 268

页数：8

共 53 条

[1] INHIBITION OF HUMAN-PLATELET CYCLOOXYGENASE BY ALPHA-TOCOPHEROL [J].