STUDIES ON THE CORRELATION OF CHROMATOGRAPHIC BEHAVIOR OF BIOPOLYMERS BETWEEN REVERSED-PHASE AND HYDROPHOBIC INTERACTION CHROMATOGRAPHY - TESTING THE STOICHIOMETRIC DISPLACEMENT BETWEEN BIOPOLYMER AND DISPLACING AGENT

被引：6

作者：

CHANG, J ^{[1
]}

GUO, L ^{[1
]}

FENG, W ^{[1
]}

GENG, X ^{[1
]}

机构：

[1] NW UNIV XIAN,DEPT CHEM,MODERN SEPARAT SCI LAB,XIAN 710069,PEOPLES R CHINA

来源：

CHROMATOGRAPHIA | 1992年 / 34卷 / 11-12期

关键词：

REVERSED-PHASE CHROMATOGRAPHY; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; STOICHIOMETRIC DISPLACEMENT MODEL FOR RETENTION; RETENTION MECHANISM;

D O I：

10.1007/BF02269868

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

One hundred and one sets of retention data obtained from twenty types of chromatographic systems with seventeen kinds of biopolymers in both reversed-phase and hydrophobic interaction chromatography were used to test the broad applications of stoichiometric displacement model of retention. We also investigated the linear relationship between the stoichiometric displacement parameter Z and molecular weight of proteins and found out that it really depends on the changes in the molecular conformation or the completeness of losing quarternary or tertiary structure of proteins during the chromatographic process.

引用

页码：589 / 596

页数：8

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