REGULATION OF THE STAPHYLOCOCCUS-AUREUS PLASMID-PI258 MERCURY RESISTANCE OPERON

被引：22

作者：

CHU, L ^{[1
]}

MUKHOPADHYAY, D ^{[1
]}

YU, HG ^{[1
]}

KIM, KS ^{[1
]}

MISRA, TK ^{[1
]}

机构：

[1] UNIV ILLINOIS,COLL MED,DEPT MICROBIOL & IMMUNOL,CHICAGO,IL 60680

来源：

JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 21期

关键词：

D O I：

10.1128/JB.174.21.7044-7047.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Experiments involving fusion between the Staphylococcus aureus plasmid pI258-encoded mer operon and the reporter gene beta-lactamase, mutational analysis, and trans-complementation studies have shown that the merR gene of pI258, which shows DNA sequence similarity with known merR genes from other bacteria, regulates the expression of the mer operon in vivo. The merR gene product is a trans-acting protein that activates mer operon transcription in the presence of the inducers Hg2+ and Cd2+. A glutathione-S-transferase-MerR fusion protein specifically bound and protected a 27-nucleotide operator sequence from DNase I digestion. This operator sequence is highly homologous with mer operator sequences of other known systems.

引用

页码：7044 / 7047

页数：4

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