DNA PROBES REVEAL RELATEDNESS OF RICE YELLOW DWARF MYCOPLASMALIKE ORGANISMS (MLOS) AND DISTINGUISH THEM FROM OTHER MLOS

被引：23

作者：

NAKASHIMA, K ^{[1
]}

KATO, S ^{[1
]}

IWANAMI, S ^{[1
]}

MURATA, N ^{[1
]}

机构：

[1] NATL AGR RES CTR, TSUKUBA, IBARAKI 305, JAPAN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 04期

关键词：

D O I：

10.1128/AEM.59.4.1206-1212.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

DNA of 10 lines of rice yellow dwarf (RYD) mycoplasmalike organisms (MLOs) from Japan, the Philippines, and Thailand hybridized with four probes containing chromosomal and six probes containing extrachromosomal DNA of a Tochigi (japan) line of RYD MLO. One chromosomal probe (RYD9) and all six extrachromosomal probes hybridized only with DNA from rice infected with RYD MLOs. Three other chromosomal probes hybridized with various other MLOs (sugarcane white leaf, onion yellows, cineraria witches'-broom, Japanese hornwort witches'-broom, water dropwort witches'-broom, gentian witches'-broom, udo dwarf, tsuwabuki witches'-broom, pelargonium witches'-broom, peach western-X, and pear decline). DNA from the culturable mollicutes Spiroplasma kunkelii, Spiroplasma citri, Mycoplasma hominis, and Mycoplasma orale did not hybridize with RYD MLO probes. The extrachromosomal DNAs hybridizing with the probes showed variations in electrophoretic behavior.

引用

页码：1206 / 1212

页数：7

共 44 条

[1] DETECTION OF DNA OF PLANT PATHOGENIC MYCOPLASMALIKE ORGANISMS BY A POLYMERASE CHAIN-REACTION THAT AMPLIFIES A SEQUENCE OF THE 16S RNA GENE [J].