COUPLED-COLUMN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF 1-HYDROXYPYRENE IN URINE OF SUBJECTS EXPOSED TO POLYCYCLIC AROMATIC-HYDROCARBONS

被引：62

作者：

BOOS, KS ^{[1
]}

LINTELMANN, J ^{[1
]}

KETTRUP, A ^{[1
]}

机构：

[1] GSF MUNICH,UMWELT & GESUNDHEIT FORSCHUNGSZENTRUM,INST OKOL CHEM,W-8042 NEUHERBERG,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 600卷 / 02期

关键词：

D O I：

10.1016/0021-9673(92)85547-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A coupled-column high-performance liquid chromatographic system for integrated, on-line sample processing and the determination of free and conjugated 1-hydroxypyrene in urine has been developed. The method is based on a "tailor-made" copper phthalocyanine-modified porous-glass precolumn packing material, which allows a direct and repeated injection of urine samples and a selective enrichment of trace amounts of particular components. The fully automated method has a low detection limit (0.01 pmol), a quantitative and matrix-independent recovery and a highly reliability, as shown by an interlaboratory comparison of methods.

引用

页码：189 / 194

页数：6

共 10 条

[1]

Angerer J, 1991, ANAL HAZARDOUS SUBST, V3, P151

[2]

GIESERT M, 1988, FRESEN Z ANAL CHEM, V330, P437

[3] ADSORPTION OF MUTAGENS TO COTTON BEARING COVALENTLY BOUND TRISULFO-COPPER-PHTHALOCYANINE [J].

HAYATSU, H ;

OKA, T ;

WAKATA, A ;

OHARA, Y ;

HAYATSU, T ;

KOBAYASHI, H ;

ARIMOTO, S .

MUTATION RESEARCH, 1983, 119 (3-4) :233-238

[4]

HAYATSU H, 1986, CHEM PHARM BULL, V34, P944

[5] 1-HYDROXYPYRENE IN URINE AS A BIOLOGICAL INDICATOR OF EXPOSURE TO POLYCYCLIC AROMATIC-HYDROCARBONS IN SEVERAL WORK ENVIRONMENTS [J].

JONGENEELEN, FJ ;

ANZION, RBM ;

SCHEEPERS, PTJ ;

BOS, RP ;

HENDERSON, PT ;

NIJENHUIS, EH ;

VEENSTRA, SJ ;

BROUNS, RME ;

WINKES, A .

ANNALS OF OCCUPATIONAL HYGIENE, 1988, 32 (01) :35-43

[6] 1-HYDROXYPYRENE IN HUMAN-URINE AFTER EXPOSURE TO COAL-TAR AND A COAL-TAR DERIVED PRODUCT [J].

JONGENEELEN, FJ ;

ANZION, RBM ;

LEIJDEKKERS, CM ;

BOS, RP ;

HENDERSON, PT .

INTERNATIONAL ARCHIVES OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH, 1985, 57 (01) :47-55

[7] BIOLOGICAL MONITORING OF POLYCYCLIC AROMATIC-HYDROCARBONS - METABOLITES IN URINE [J].

JONGENEELEN, FJ ;

BOS, RP ;

ANZION, RBM ;

THEUWS, JLG ;

HENDERSON, PT .

SCANDINAVIAN JOURNAL OF WORK ENVIRONMENT & HEALTH, 1986, 12 (02) :137-143

[8] DETERMINATION OF HYDROXYLATED METABOLITES OF POLYCYCLIC AROMATIC-HYDROCARBONS IN URINE [J].

JONGENEELEN, FJ ;

ANZION, RBM ;

HENDERSON, PT .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 413 :227-232

[9] ANALYSES OF TRACES OF POLYCYCLIC AROMATIC-HYDROCARBONS BY ADSORPTION ON COBALT PHTHALOCYANINE AND BARIUM SALTS OF SULFOPHTHALOCYANINES FOLLOWED BY THERMAL-DESORPTION GAS-CHROMATOGRAPHY [J].

KUSUDA, K ;

SHIRAKI, K ;

MIWA, T .

ANALYTICA CHIMICA ACTA, 1989, 224 (01) :1-11

[10]

LINTELMANN J, 1990, THESIS U PADERBORN P

← 1 →