SCALE-UP OF RECOMBINANT PROTEIN-PURIFICATION BY HYDROPHOBIC INTERACTION CHROMATOGRAPHY

被引：10

作者：

VORAUER, K ^{[1
]}

SKIAS, M ^{[1
]}

TRKOLA, A ^{[1
]}

SCHULZ, P ^{[1
]}

JUNGBAUER, A ^{[1
]}

机构：

[1] UNIV WIEN,INST ANGEW MIKROBIOL,NUSSDORFERLANDE 11,A-1190 VIENNA,AUSTRIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 625卷 / 01期

关键词：

D O I：

10.1016/0021-9673(92)87218-W

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The scale-up of hydrophobic interaction chromatography is described. Human recombinant superoxide dismutase was used as a model. The scale-up was performed by keeping the height to diameter (H/D) ratio of the column constant. The success of scale-up was evaluated by reversed-phase high-performance liquid chromatography of the eluted material. The wrong H/D ratio causes decreased resolution.

引用

页码：33 / 39

页数：7

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