STRATEGIES FOR ONLINE COUPLING OF IMMUNOASSAYS TO HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

On-line coupling of reversed-phase high-performance liquid chromatography (HPLC) with bioassays, such as immunoassays, allows a combination of the separation power and automation potential of LC with the high selectivity and sensitivity of biochemical detection principles, In this way important problems related to LC and batch immunoassays, i.e., the lack of selective and sensitive detection methods on the one hand and the interference of cross-reactive matrix components on the other hand, can be overcome, This article presents different strategies to implement immunochemical interactions in on-line post-column detection techniques: either labelled antibodies or labelled antigens can be used to detect the presence of antigens eluting from the reversed-phase LC column.