A THERMOSTABLE HYDROGEN-PEROXIDE SENSOR-BASED ON WIRING OF SOYBEAN PEROXIDASE

被引：55

作者：

VREEKE, MS ^{[1
]}

YONG, KT ^{[1
]}

HELLER, A ^{[1
]}

机构：

[1] UNIV TEXAS,DEPT CHEM ENGN,AUSTIN,TX 78712

来源：

ANALYTICAL CHEMISTRY | 1995年 / 67卷 / 23期

关键词：

D O I：

10.1021/ac00119a007

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A thermostable soybean peroxidase-based biosensor was formed by cross-linking and electrically ''wiring'' the enzyme through a redox-conducting hydrogel to a glassy carbon electrode. At 65 degrees C the sensitivity of the sensor decayed only at a rate of only <2%/h. The sensor maintained, at 25 degrees C, the 1 A cm(-2) M(-1) sensitivity and the 0.1-200 mu M dynamic range of the earlier ''wired'' horseradish peroxidase-based sensors.

引用

页码：4247 / 4249

页数：3

共 19 条

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