ULTRASENSITIVE ENZYMATIC RADIOIMMUNOASSAY - APPLICATION TO DETECTION OF CHOLERA TOXIN AND ROTAVIRUS

被引：105

作者：

HARRIS, CC

YOLKEN, RH

KROKAN, H

HSU, IC

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT PEDIAT,BALTIMORE,MD 21205

[2] UNIV TROMSO,INST MED BIOL,N-9001 TROMSO,NORWAY

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 10期

关键词：

D O I：

10.1073/pnas.76.10.5336

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Rotavirus and enterotoxin-producing bacteria are major causes of diarrheal disease in humans. A method of rapid diagnosis, ultrasensitive enzymatic radioimmunoassay, has been developed to quantitatively detect cholera toxin and rotavirus. The method uses features of both enzyme-linked immunosorbent assay and radioimmunoassay; however, the sensitivity of the assay is 100- to 1000-fold more sensitive than the two parent assays. Ultrasensitive enzymatic radioimmunoassay should also be useful in measuring other biologically important agents such as drugs and hormones.

引用

页码：5336 / 5339

页数：4

共 17 条

[1] SIMPLIFIED RADIOIMMUNOASSAY FOR DETECTION OF HUMAN ROTAVIRUS IN STOOLS
CUKOR, G
BERRY, MK
BLACKLOW, NR
[J]. JOURNAL OF INFECTIOUS DISEASES, 1978, 138 (06) : 906 - 910
[2] ENGVALL E, 1972, J IMMUNOL, V109, P129
[3] ENGVALL E, 1976, 1ST INT S IMM TECHN, P135
[4] FEBER JP, 1978, ADV CLIN CHEM, V20, P130
[5] Fernley H.N., 1971, ENZYMES, V4, P417
[6] Nahmias A J, 1970, Adv Pediatr, V17, P185
[7] SACK RB, 1977, J CLIN MICROBIOL, V5, P570
[8] Thorell JL, 1978, RADIOIMMUNOASSAY REL
[9] VOLLER A, 1976, B WORLD HEALTH ORGAN, V53, P55
[10] WISDOM GB, 1976, CLIN CHEM, V22, P1243

← 1 2 →