A facile system was developed for the quantitative determination of lipase regioselectivities in organic solvents towards the 1(3)-position of glycerides. It was utilized for the measurement of the regioselectivities displayed by lipase preparations from Mucor miehei (Lipozyme(TM)), Pseudomonas fluorescens and Rhizopus delemar. It was shown that the lipases from Mucor miehei and Pseudomonas fluorescens do in fact not display the high 1(3)-specifities reported in the literature for these enzymes.