INVITRO AND INVIVO DETECTION OF SOMATOSTATIN RECEPTORS IN HUMAN-MALIGNANT LYMPHOMAS

被引:118
作者
REUBI, JC
WASER, B
VANHAGEN, M
LAMBERTS, SWJ
KRENNING, EP
GEBBERS, JO
LAISSUE, JA
机构
[1] UNIV BERN,INST PATHOL,CH-3000 BERN,SWITZERLAND
[2] ERASMUS UNIV,DEPT INTERNAL MED,3000 DR ROTTERDAM,NETHERLANDS
[3] ERASMUS UNIV,DEPT NUCL MED,3000 DR ROTTERDAM,NETHERLANDS
[4] KANTONSSPITAL,INST PATHOL,CH-6004 LUCERNE,SWITZERLAND
关键词
D O I
10.1002/ijc.2910500613
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
A wide variety of primary and metastatic human neoplasms express somatostatin receptors (SS-Rs). We evaluated the SS-R status of malignant lymphomas that had been surgically removed from 31 patients, by use of in vitro SS-R autoradiography with the SS analog I-125-[Tyr3]-octreotide as radioligand. Of 11 low-grade-malignancy B-cell non-Hodgkin's lymphomas, 10 were SS-R-positive, with a high receptor density restricted to the neoplastic follicles. All of the 8 intermediate-grade lymphomas were SS-R-positive. Of the B-cell lymphomas of high-grade malignancy, 7 out of 10 were SS-R-positive, often with a high density of receptors. One T-cell lymphoma and one Hodgkin's lymphoma were also positive. SS-Rs were of high affinity (K(D) = 1.2 nM) and specific for bioactive SS analogs. In 4 patients, the lymphomas were localized in vivo by use of gamma-camera scintigraphy after i.v. injection of the SS analog In-111-[DTPA-D-Phe1]-octreotide. Hot spots, identified in all 4 patients, corresponded to SS-R-positive malignant-lymphoma tissue, as confirmed by receptor autoradiography of the surgically removed tumors. Our data show that SS-Rs are valuable pathobiochemical tissue markers and potentially useful in vivo diagnostic tools for human malignant lymphomas.
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页码:895 / 900
页数:6
相关论文
共 36 条
[1]  
AKTORIES K, 1983, MOL PHARMACOL, V24, P183
[2]  
[Anonymous], 1982, CANCER, V49, P2112
[3]  
BAKKER W H, 1991, Journal of Nuclear Medicine, V32, P1084
[4]  
BAKKER WH, 1990, J NUCL MED, V31, P1501
[5]   INVIVO APPLICATION OF [IN-111-DTPA-D-PHE1]-OCTREOTIDE FOR DETECTION OF SOMATOSTATIN RECEPTOR-POSITIVE TUMORS IN RATS [J].
BAKKER, WH ;
KRENNING, EP ;
REUBI, JC ;
BREEMAN, WAP ;
SETYONOHAN, B ;
DEJONG, M ;
KOOIJ, PPM ;
BRUNS, C ;
VANHAGEN, PM ;
MARBACH, P ;
VISSER, TJ ;
PLESS, J ;
LAMBERTS, SWJ .
LIFE SCIENCES, 1991, 49 (22) :1593-1601
[6]   IDENTIFICATION OF HUMAN MONONUCLEAR LEUKOCYTES BEARING RECEPTORS FOR SOMATOSTATIN AND GLUCAGON [J].
BHATHENA, SJ ;
LOUIE, J ;
SCHECHTER, GP ;
REDMAN, RS ;
WAHL, L ;
RECANT, L .
DIABETES, 1981, 30 (02) :127-131
[7]   CHARACTERISTICS AND DISTRIBUTION OF RECEPTORS FOR [D-TRP6]-LUTEINIZING HORMONE-RELEASING HORMONE, SOMATOSTATIN, EPIDERMAL GROWTH-FACTOR, AND SEX STEROIDS IN 500 BIOPSY SAMPLES OF HUMAN-BREAST CANCER [J].
FEKETE, M ;
WITTLIFF, JL ;
SCHALLY, AV .
JOURNAL OF CLINICAL LABORATORY ANALYSIS, 1989, 3 (03) :137-147
[8]  
FOEKENS JA, 1989, CANCER RES, V49, P7002
[9]   USE OF AVIDIN-BIOTIN-PEROXIDASE COMPLEX (ABC) IN IMMUNOPEROXIDASE TECHNIQUES - A COMPARISON BETWEEN ABC AND UNLABELED ANTIBODY (PAP) PROCEDURES [J].
HSU, SM ;
RAINE, L ;
FANGER, H .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1981, 29 (04) :577-580
[10]   GASTROINTESTINAL REGULATORY PEPTIDES MODULATE MOUSE LYMPHOCYTE FUNCTIONS UNDER SERUM-FREE CONDITIONS INVITRO [J].
KRCO, CJ ;
GORES, A ;
GO, VLW .
IMMUNOLOGICAL INVESTIGATIONS, 1986, 15 (02) :103-111