CLONING, SEQUENCING, AND TRANSCRIPTIONAL ANALYSIS OF THE GENE CODING FOR THE VEGETATIVE SIGMA-FACTOR OF AGROBACTERIUM-TUMEFACIENS

被引：11

作者：

SEGAL, G ^{[1
]}

RON, EZ ^{[1
]}

机构：

[1] TEL AVIV UNIV, GEORGE S WISE FAC LIFE SCI, DEPT MOLEC MICROBIOL & BIOTECHNOL, IL-6678 TEL AVIV, ISRAEL

来源：

JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 10期

关键词：

D O I：

10.1128/JB.175.10.3026-3030.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The sigA gene of Agrobacterium tumefaciens was cloned and sequenced. Comparison with previously analyzed sigA genes revealed a high degree of similarity in nucleotide and amino acid sequences of regions two, three, and four of vegetative sigma factors. However, the upstream regulatory region shows no sequence homology with the Escherichia coli heat shock (sigma32) promoters. It also does not contain the hairpin-loop structure (inverted repeat sequence) that was found in the upstream region of the groE operon in A. tumefaciens. The transcription initiation site of the gene was determined and found to be at the same position during normal growth and under heat shock conditions. Furthermore, no heat shock activation was observed at the transcriptional level.

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页码：3026 / 3030

页数：5

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