GLUTAMINE-SYNTHETASE ISOFORMS APPEARING IN SUNFLOWER COTYLEDONS DURING GERMINATION - EFFECTS OF LIGHT AND NITRATE

被引：27

作者：

DELAHABA, P ^{[1
]}

CABELLO, P ^{[1
]}

MALDONADO, JM ^{[1
]}

机构：

[1] UNIV CORDOBA,FAC CIENCIAS,DIV FISIOL VEGETAL,DEPT BIOL VEGETAL & ECOL,AVDA SAN ALBERTO MAGNO,E-14004 CORDOBA,SPAIN

来源：

PLANTA | 1992年 / 186卷 / 04期

关键词：

AMMONIA AMMONIUM ASSIMILATION; ENZYME REGULATION; GLUTAMINE SYNTHETASE; HELIANTHUS (GLUTAMINE SYNTHETASE); SEED GERMINATION;

D O I：

10.1007/BF00198038

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ion-exchange chromatography has been used to separate the isoforms of glutamine synthetase (GS; EC 6.3.1.2) appearing in sunflower (Helianthus annuus L. cv. Peredovic) cotyledons during seedling growth under different light and nitrogen conditions. Both in dry and imbibed seeds. only a single form of GS (GS(s)) was detected. Upon seed germination, the GS(s) isoform was gradually replaced by cytosolic (GS1) and plastidic (GS2) isoforms. Light and nitrate decreased the levels of GS1. In contrast, the appearance of GS, was greatly stimulated by light. Nitrate also had a positive effect, particularly in the light. Light and nitrate acted synergistically on the appearance of GS2. The GS2:GS1 ratio in cotyledons of 9-d-old seedlings ranged from about 2, in darkness and nitrate-deprivation conditions, to 16 under light and nitrate application. The possible physiological roles of the distinct GS isoforms appearing in the epigeal cotyledons of sunflower during germination, and their differential regulation by light and nitrate, are discussed.

引用

页码：577 / 581

页数：5

共 21 条

[1] AGUERA E, 1987, PLANT PHYSIOL, V6, P255
[2] GLUTAMINE-SYNTHETASE ISOENZYMES OF PHASEOLUS-VULGARIS L - SUBUNIT COMPOSITION IN DEVELOPING ROOT-NODULES AND PLUMULES
BENNETT, MJ
CULLIMORE, JV
[J]. PLANTA, 1989, 179 (04) : 433 - 440
[3] ISOFORMS OF GLUTAMINE-SYNTHETASE IN COTYLEDONS, LEAVES AND ROOTS OF SUNFLOWER PLANTS
CABELLO, P
DELAHABA, P
MALDONADO, JM
[J]. JOURNAL OF PLANT PHYSIOLOGY, 1991, 137 (03) : 378 - 380
[4] MOLECULAR APPROACHES TO THE STUDY OF AMINO-ACID BIOSYNTHESIS IN PLANTS
CORUZZI, GM
[J]. PLANT SCIENCE, 1991, 74 (02) : 145 - 155
[5] THE MOLECULAR-BIOLOGY AND BIOCHEMISTRY OF PLANT GLUTAMINE-SYNTHETASE FROM ROOT-NODULES OF PHASEOLUS-VULGARIS L AND OTHER LEGUMES
CULLIMORE, JV
BENNETT, MJ
[J]. JOURNAL OF PLANT PHYSIOLOGY, 1988, 132 (04) : 387 - 393
[6] DEVELOPMENT OF NITROGEN-ASSIMILATING ENZYMES IN SUNFLOWER COTYLEDONS DURING GERMINATION AS AFFECTED BY THE EXOGENOUS NITROGEN-SOURCE
DELAHABA, P
AGUERA, E
MALDONADO, JM
[J]. PLANTA, 1988, 173 (01) : 52 - 57
[7] PHOTORESPIRATION AND LIGHT ACT IN CONCERT TO REGULATE THE EXPRESSION OF THE NUCLEAR GENE FOR CHLOROPLAST GLUTAMINE-SYNTHETASE
EDWARDS, JW
CORUZZI, GM
[J]. PLANT CELL, 1989, 1 (02) : 241 - 248
[8] HEWITT EJ, 1966, COMMONWEALTH BUREAU, V22
[9] EVIDENCE FOR A CYTOSOLIC-DEPENDENT LIGHT INDUCTION OF CHLOROPLASTIC GLUTAMINE-SYNTHETASE DURING GREENING OF ETIOLATED RICE LEAVES
HIREL, B
VIDAL, J
GADAL, P
[J]. PLANTA, 1982, 155 (01) : 17 - 23
[10] Lea P.J., 1990, BIOCH PLANTS, V16, P121, DOI 10.1016/B978-0-08-092616-2.50010-3

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