HIGHLY EFFICIENT DNA-SYNTHESIS IN ISOLATED-MITOCHONDRIA FROM RAT-LIVER

被引：38

作者：

ENRIQUEZ, JA ^{[1
]}

RAMOS, J ^{[1
]}

PEREZMARTOS, A ^{[1
]}

LOPEZPEREZ, MJ ^{[1
]}

MONTOYA, J ^{[1
]}

机构：

[1] UNIV ZARAGOZA,FAC VET,DEPT BIOQUIM & BIOL MOLEC & CELULAR,E-50013 ZARAGOZA,SPAIN

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 10期

关键词：

D O I：

10.1093/nar/22.10.1861

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a highly efficient DNA-synthesizing system with isolated intact rat liver mitochondria. The ATP requirements for this in organello DNA synthesis are provided by endogenous synthesis in the presence of exogenous ADP and an oxidizable substrate. In this system, mitochondrial DNA synthesis strikingly proceeds at a constant rate for about 5 h at 37 degrees C. Gel electrophoresis, hybridization and restriction enzyme analyses show that intact mitochondria synthesize nucleic acids with a size of 16.5 kb, that correspond to mitochondrial DNA, and that both DNA strands are replicated. This in organello DNA synthesis requires the supply of dNTPs and decreases at high ADP concentration in the incubation medium.

引用

页码：1861 / 1865

页数：5

共 20 条

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