IFN-ALPHA INDUCES MXA GENE-EXPRESSION IN CULTURED HUMAN CORNEAL FIBROBLASTS

被引：8

作者：

ABRAMS, AE

BALISH, MJ

BRANDT, CR

机构：

[1] UNIV WISCONSIN,SCH MED,DEPT OPHTHALMOL & VISUAL SCI,MADISON,WI 53706

[2] UNIV WISCONSIN,SCH MED,DEPT MED MICROBIOL & IMMUNOL,MADISON,WI 53706

来源：

EXPERIMENTAL EYE RESEARCH | 1995年 / 60卷 / 02期

关键词：

MXA; INTERFERON-ALPHA; HUMAN CORNEAL FIBROBLASTS;

D O I：

10.1016/S0014-4835(95)80003-4

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Treatment of certain human cells with Interferon-alpha (IFN-alpha) induces the synthesis of a 76 kDa protein designated MxA that is involved in resistance to viral infection. We have used a specific cDNA clone and monoclonal Ab to show that MxA is induced in IFN-alpha treated human corneal fibroblast cultures. Mx RNA was increased 23-fold and 45-fold after 5 and 9 h of IFN-alpha treatment, respectively. The MxA protein was detectable by immunoblotting at 5 hr after IFN treatment and peaked at 17 hr. Concentrations of IFN-alpha as low as 1 U/ml induced detectable amounts of MxA, and expression was maximal at 1 x 10(3) U ml(-1). These results confirm that MxA synthesis is induced in human corneal fibroblasts treated with IFN-alpha.

引用

页码：137 / 142

页数：6

共 34 条

[1] TRANSGENIC MICE WITH INTRACELLULAR IMMUNITY TO INFLUENZA-VIRUS [J].