NONAQUEOUS BEEF FLAVOR COMPONENTS . COMPOSITION OF PETROLEUM ETHER-EXTRACTABLE INTRAMUSCULAR POLAR LIPIDS

Petroleum ether-extractable intramuscular lipids were separated by silica gel chromatography into polar and neutral lipids. Polar lipids were fractionated on an anion exchange DEAE-cellulose column into at least 11 components. Thin-layer chromatography Rf values, staining behavior with specific reagents, and infrared spectra of intact lipids enabled the identification of seven polar lipids. Six of these were positively identified as phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin, and phosphatidylinositol; the seventh compound was tentatively identified as sulfatide. © 1969, American Chemical Society. All rights reserved.