ISOLATION AND ELECTROPHORETIC PROPERTIES OF A CALCIUM-BINDING PROTEIN FROM THE CILIATE TETRAHYMENA-PYRIFORMIS

被引：41

作者：

SUZUKI, Y ^{[1
]}

HIRABAYASHI, T ^{[1
]}

WATANABE, Y ^{[1
]}

机构：

[1] UNIV TSUKUBA, INST BIOL SCI, NIIHARI, IBARAKI 30031, JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 01期

关键词：

D O I：

10.1016/0006-291X(79)91618-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A calcium-binding protein (TCBP) was isolated from the ciliate Tetrahymena pyriformis by a procedure which included 8 M urea extraction of the cell homogenate or boiling water extraction of acetone-dried powder of the cell, ammonium sulfate precipitation (60-80% saturation) and preparative electrophoresis on alkaline polyacrylamide gel. The TCBP has a molecular weight of 14,000 daltons and an isoelectric point of pH 4.0 and is easily detected in crude cell extracts by the change of electrophoretic mobility depending on Ca ion concentration. By equilibrium dialysis TCBP was found to bind to 2 moles of calcium per mole of the protein at a free calcium concentration of 10-4 M. On alkaline polyacrylamide gel, the TCBP interacts with rabbit skeletal muscle troponin I and with some material(s) of Tetrahymena origin to form Ca2+-dependent complexes. © 1979.

引用

页码：253 / 260

页数：8

共 26 条

[1]

AMOS WB, 1975, J CELL SCI, V19, P203

[2] EFFECT OF TROPONIN C-LIKE PROTEIN FROM BOVINE BRAIN (BRAIN MODULATOR PROTEIN) ON MG-2+-STIMULATED ATPASE OF SKELETAL-MUSCLE ACTOMYOSIN [J].

AMPHLETT, GW ;

VANAMAN, TC ;

PERRY, SV .

FEBS LETTERS, 1976, 72 (01) :163-168

[3] IDENTIFICATION OF A CALCIUM-BINDING PROTEIN AS A CALCIUM-DEPENDENT REGULATOR OF BRAIN ADENYLATE CYCLASE [J].

BROSTROM, CO ;

HUANG, YC ;

BRECKENRIDGE, BM ;

WOLFF, DJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (01) :64-68

[4] CYCLIC 3,5-NUCLEOTIDE PHOSPHODIESTERASE . DEMONSTRATION OF AN ACTIVATOR [J].

CHEUNG, WY .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1970, 38 (03) :533-&

[5] SUBUNITS AND BIOLOGICAL-ACTIVITY OF POLYMORPHIC FORMS OF TROPOMYOSIN [J].

CUMMINS, P ;

PERRY, SV .

BIOCHEMICAL JOURNAL, 1973, 133 (04) :765-+

[6] CONTROL OF CONTRACTILITY IN SPIROSTOMUM BY DISSOCIATED CALCIUM IONS [J].

ETTIENNE, EM .

JOURNAL OF GENERAL PHYSIOLOGY, 1970, 56 (02) :168-&

[7] CALCIUM-DEPENDENT PROTEIN ACTIVATOR OF MAMMALIAN CYCLIC NUCLEOTIDE PHOSPHODIESTERASE FROM BLASTOCLADIELLA-EMERSONII [J].

GOMES, SL ;

MAIA, JCDC ;

MENNUCCI, L .

FEBS LETTERS, 1979, 99 (01) :39-42

[8] CALCIUM BINDING BY TROPONIN COMPLEX, AND PURIFICATION AND PROPERTIES OF TROPONIN-A [J].

HARTSHORNE, DJ ;

PYUN, HY .

BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 229 (03) :698-+

[9] CALCIUM-BINDING TO BOVINE BRAIN TUBULIN [J].

HAYASHI, M ;

MATSUMURA, F .

FEBS LETTERS, 1975, 58 (01) :222-225

[10] INTERACTION OF CALCIUM-BINDING PROTEIN (TROPONIN C) WITH BIVALENT-CATIONS AND INHIBITORY PROTEIN (TROPONIN I) [J].

HEAD, JF ;

PERRY, SV .

BIOCHEMICAL JOURNAL, 1974, 137 (02) :145-+

← 1 2 3 →