NEW METHOD OF IODINATING COLLAGENS FOR USE IN RADIOIMMUNOASSAY

被引:45
作者
ROLL, FJ
MADRI, JA
FURTHMAYR, H
机构
[1] Department of Pathology, Yale University School of Medicine, New Haven, CT 06510
关键词
D O I
10.1016/0003-2697(79)90611-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Purified collagens from a variety of species were iodinated to a high specific activity with the N-hydroxysuccinimide ester of I125-labeled p-hydroxyphenyl propionic acid (Bolton-Hunter reagent). Labeling had no effect on the immunoreactivity of the collagen as determined by hemagglutination inhibition. This compound presumably acylates the abundant ε{lunate}-amino groups of lysyl and hydroxylysyl residues in the collagen molecule. Using this method it is possible to label pepsin-extracted collagen from which the terminal nonhelical extensions containing tyrosine have been cleaved. The application of Bolton-Hunter-labeled collagens to radioimmunoassay of affinity-purfied antibodies against collagen is demonstrated. © 1979.
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页码:489 / 499
页数:11
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