SEPARATION OF POLYPEPTIDE-CHAINS OF RICIN AND THE INTERACTION OF THE A-CHAIN WITH CIBACRON BLUE-F3GA

被引：25

作者：

APPUKUTTAN, PS

BACHHAWAT, BK

机构：

[1] Indian Institute of Experimental Medicine, Calcutta

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 580卷 / 01期

关键词：

Cibacron Blue F[!sub]3[!/sub]GA; Galactomannan; Nucleotide-binding domain; Ricin polypeptide chain;

D O I：

10.1016/0005-2795(79)90192-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

From ricin bound to the galactomannan guar gum in a column, the nonbinding toxic A chain could be eluted by reduction with 2-mercaptoethanol and later the B chain by lactose. The presence of a nucleotide-binding domain on the toxic chain A could be demonstrated from its interaction with the blue dye Cibacron Blue F3GA. © 1979.

引用

页码：10 / 14

页数：5

共 11 条

[1] APPUKUTTAN PS, 1977, INDIAN J BIOCHEM BIO, V14, P382
[2] Easterday R L, 1974, Adv Exp Med Biol, V42, P123
[3] INTERACTION OF RICINUS-COMMUNIS AGGLUTININ WITH NORMAL AND TUMOR-CELL SURFACES
NICOLSON, GL
BLAUSTEIN, J
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 266 (02) : 543 - +
[4] DIFFERENT BIOLOGICAL PROPERTIES OF 2 CONSTITUENT PEPTIDE CHAINS OF RICIN, A TOXIC PROTEIN INHIBITING PROTEIN-SYNTHESIS
OLSNES, S
PIHL, A
[J]. BIOCHEMISTRY, 1973, 12 (16) : 3121 - 3126
[5] OLSNES S, 1976, J BIOL CHEM, V251, P3985
[6] MECHANISM OF ACTION OF TOXIC LECTINS ABRIN AND RICIN
OLSNES, S
REFSNES, K
PIHL, A
[J]. NATURE, 1974, 249 (5458) : 627 - 631
[7] OLSNES S, 1976, SPECIFICITY ACTION A, P129
[8] INHIBITION BY RICIN OF PROTEIN-SYNTHESIS IN-VITRO - 60 S RIBOSOMAL-SUBUNIT AS TARGET OF TOXIN
SPERTI, S
MONTANARO, L
MATTIOLI, A
STIRPE, F
[J]. BIOCHEMICAL JOURNAL, 1973, 136 (03) : 813 - 815
[9] USE OF BLUE DEXTRAN AS A PROBE FOR NICOTINAMIDE ADENINE-DINUCLEOTIDE DOMAIN IN PROTEINS
STELLWAGEN, E
[J]. ACCOUNTS OF CHEMICAL RESEARCH, 1977, 10 (03) : 92 - 98
[10] BINDING OF CIBACRON BLUE F3GA TO PROTEINS CONTAINING DINUCLEOTIDE FOLD
THOMPSON, ST
STELLWAGEN, E
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (02) : 361 - 365

← 1 2 →