ZYMOGRAPHIC SCREENING OF PLANT PEROXIDASE ISOENZYMES OXIDIZING 4-HYDROXYSTILBENES

被引：36

作者：

CALDERON, AA ^{[1
]}

PEDRENO, MA ^{[1
]}

ROSBARCELO, A ^{[1
]}

MUNOZ, R ^{[1
]}

机构：

[1] UNIV MURCIA,DEPT PLANT BIOL,CAMPUS ESPINARDO,E-30071 MURCIA,SPAIN

来源：

ELECTROPHORESIS | 1990年 / 11卷 / 06期

关键词：

D O I：

10.1002/elps.1150110614

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A zymographic assay is described for the detection of peroxidase isoenzymes oxidizing 4‐hydroxystilbene following isoelectric focusing. The assay is based on coupling intermediate products of the oxidation of 4‐hydroxystilbene with 4‐aminoantipyrine, with resultant formation of dye complexes. Control experiments in the absence of 4‐hydroxystilbene and hydrogen peroxide demonstrate the peroxidative nature of the 4‐hydroxystilbene‐dependent dye reaction. Copyright © 1990 VCH Verlagsgesellschaft mbH

引用

页码：507 / 508

页数：2

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