DEVELOPMENTAL-CHANGES IN THE ACTIVITY AND CELLULAR-LOCALIZATION OF HEPATIC CASEIN KINASE-II IN THE RAT

被引：13

作者：

GRUPPUSO, PA ^{[1
]}

BOYLAN, JM ^{[1
]}

机构：

[1] BROWN UNIV, BIOCHEM SECT, PROVIDENCE, RI 02903 USA

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 1995年 / 58卷 / 01期

关键词：

FETUS; LIVER; HEPATOCYTES; CELL PROLIFERATION; PROTEIN PHOSPHORYLATION;

D O I：

10.1002/jcb.240580109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The activity and cellular localization of hepatic casein kinase II (CKII) was examined during late fetal development in the rat. Cultured fetal hepatocytes displayed constitutive CKII activity which was not further activated by growth factor exposure. Similarly, fetal liver CKII showed approximately fivefold greater activity than adult liver. The fetal hepatic activity was, to a large degree, localized to a nuclear fraction. Postnuclear cytosol preparations from fetal and adult liver showed similar CKII activity. In all cases, FPLC ion exchange chromatography followed by Western immunoblotting showed that immunoreactive CKII coincided with kinase activity. However, parallel determinations of CKII activity and immunoreactive CKII levels showed a higher (five- to sixfold) CKII specific activity in nuclear extracts compared to cytosol. In summary, fetal hepatic CKII demonstrates coincident nuclear localization and activation. We hypothesize that the regulation of hepatic CKII is relevant to the mitogen-independent proliferation displayed by fetal rat hepatocytes. (C) 1995 Wiley-Liss, Inc.

引用

页码：65 / 72

页数：8

共 23 条

[1] STIMULATION OF CASEIN KINASE-II BY EPIDERMAL GROWTH-FACTOR - RELATIONSHIP BETWEEN THE PHYSIOLOGICAL-ACTIVITY OF THE KINASE AND THE PHOSPHORYLATION STATE OF ITS BETA-SUBUNIT [J].