MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF HUMAN PREPROCATHEPSIN-C

被引：80

作者：

PARIS, A

STRUKELJ, B

PUNGERCAR, J

RENKO, M

DOLENC, I

TURK, V

机构：

[1] Department of Biochemistry and Molecular Biology, Jožef Stefan Institute, 61000 Ljubljana

来源：

FEBS LETTERS | 1995年 / 369卷 / 2-3期

关键词：

PREPROCATHEPSIN C; ILEUM (HUMAN); CDNA CLONING; CYSTEINE PROTEINASE;

D O I：

10.1016/0014-5793(95)00777-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA clone (C1) coding for human preprocathepsin C was isolated from a human ileum cDNA library using a rat kidney-derived RT-PCR probe and its complete nucleotide sequence determined. The full-length 1857 bp sequence codes for a protein of 463 amino acid residues with a calculated molecular mass of 51848 Da. Comparison of the deduced amino acid sequence with that of rat preprocathepsin C indicates an 87.5% identity. A multiple alignment of the deduced cathepsin C sequence of 233 residues which, by analogy to other cysteine proteinases, corresponds to the mature protein, confirms that human cathepsin C belongs to tbe papain superfamily.

引用

页码：326 / 330

页数：5

共 31 条

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