DISTRIBUTION PATTERN AND ENZYMIC HYPERMETHYLATION OF INVERTED REPETITIVE DNA SEQUENCES IN P815-MASTOCYTOMA CELLS

被引：25

作者：

DRAHOVSKY, D ^{[1
]}

BOEHM, TLJ ^{[1
]}

KREIS, W ^{[1
]}

机构：

[1] MEM SLOAN KETTERING CANC CTR,NEW YORK,NY 10021

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 563卷 / 01期

基金：

美国国家卫生研究院;

关键词：

(Mastocytoma cell); DNA methylation; Repetitive DNA;

D O I：

10.1016/0005-2787(79)90004-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A specific class of DNA sequences, the inverted repetitive sequences, forms a double-stranded structure within a single linear polynucleotide chain in denatured DNA. The reassociation process is unimolecular and occurs very fast. Quantitative analyses have shown that in mouse P815 cells these sequences comprise about 4% of the nuclear DNA and are interspersed within sequences of other degrees of repetitiveness. After labeling the cells with l-[Me-3H]methionine and [14C]deoxycytidine, relative rates of enzymic DNA methylation were computed on the basis of radioactivities found in pyrimidine residues of the nuclear DNA. The results indicate that in P815 cells, DNA of inverted repetitive sequences is methylated to a level about 50% higher than the normal repetitive DNA sequences and to about 300% higher than the unique and intermediary sequences. The biological function of the inverted repetitive sequences, as well as of the role of enzymic methylation of DNA remains unknown. © 1979.

引用

页码：28 / 35

页数：8

共 42 条

[1] NEWLY SYNTHESIZED DNA IS NOT METHYLATED [J].