METABOLIC CARBOHYDRATE-LABELING OF GLYCOLIPIDS FROM MOUSE SPLENOCYTES - MITOGEN-STIMULATED B-CELL AND T-CELL - SHOW DIFFERENT LABELING PATTERNS

Splenic lymphocytes from CBA/J, AKR/A/J, BALB/c/A, C57/BL/6J, C3H/HeJ and C3H/Tif nu/nu mice and B lymphocyte or T lymphocyte preparations derived from CBA/J mouse spleen were cultivated in the presence of either concanavalin A, phytohemagglutinin, Salmonella minnesotaR595 lipopolysaccharide or Proteus mirabilis soluble lipoprotein. The mitogens stimulated the incorporation of [14C]galactose into acid‐insoluble cell material with the same specificity for B or T cells as that known for thymidine incorporation. The glycolipids extracted from mitogen‐activated, carbohydrate‐labelled B or T cells were compared by thin‐layer chromatography and characteristic differences between B and T cells were noted in the ganglioside as well as in the neutral glycolipid fractions. In addition, subsets of B or T cells, namely lipopolysaccharide‐responsive or lipoprotein‐responsive B‐cell populations or nylon‐purified T cells may be recognized by characteristic neutral glycolipid bands. Copyright © 1979, Wiley Blackwell. All rights reserved